Trypticase™ Soy with 5% Sheep Blood/CLED Agar
Overview
"Trypticase™ Soy Agar with 5% Sheep Blood: TrypticaseTM Soy Agar is a widely used growth medium derived from a soybean-based formula outlined in the U.S. Pharmacopeia. The inclusion of blood in this medium enhances its ability to support the growth of fastidious bacteria, those with complex nutritional requirements. Furthermore, the presence of blood allows for the observation of hemolysis, the breakdown of red blood cells. This characteristic, particularly the type of hemolysis observed, is an important tool for differentiating various bacterial species, especially those belonging to the Streptococcus genus. The absence of carbohydrates in the medium ensures that hemolysis is accurately observed and not masked by other metabolic reactions. CLED Agar: In 1960, Sandys reported on the development of a new method of preventing the swarming of Proteus on solid media by restricting the electrolytes in the culture medium. Previous chemical methods used to inhibit swarming by Proteus included the addition of chloral hydrate, alcohol, sodium azide, surface-active agents, boric acid, and sulfonamides to the culture medium. This electrolyte-deficient medium of Sandys was modified by Mackey and Sandys for use in urine culture by substituting lactose and sucrose for the mannitol and increasing the concentrations of the bromothymol blue indicator and of the agar. These two investigators further modified the medium by the incorporation of cystine to enhance the growth of cystine-dependent “dwarf colony” coliforms and by deletion of sucrose. They designated the new medium as Cystine-Lactose-Electrolyte-Deficient (CLED) medium and reported it ideal for dip-inoculum techniques and urinary bacteriology in general.
Intended Use
1st Agar: For the growth of fastidious organisms and the visualisation of hemolytic reactions 2nd Agar: For the isolation, enumeration and presumptive identification of microorganism from urine
Product Information
Category
Catalog Number
SP90T1021, SP90T1022
Pack Size
10 per pack