Mueller Hinton II Agar/Mueller Hinton Agar
Overview
Mueller Hinton Agar was originally developed for the cultivation of pathogenic Neisseria. However, these organisms are now commonly isolated on selective media. Because clinical microbiology laboratories in the early 1960s used a wide variety of procedures for determining the susceptibility of bacteria to antibiotic and chemotherapeutic agents, Bauer, Kirby and others developed a standardised procedure in which Mueller Hinton Agar was selected as the test medium. A subsequent international collaborative study confirmed the value of Mueller Hinton Agar for this purpose because of the relatively good reproducibility of the medium, the simplicity of its formula, and the wealth of experimental data accumulated using this medium. The CLSI has written a performance standard for the Bauer-Kirby procedure and this document should be consulted for additional details. The procedure is recommended for testing rapidly growing aerobic or facultatively anaerobic bacterial pathogens, such as staphylococci, members of the Enterobacteriaceae, aerobic gram-negative rods; e.g., Pseudomonas spp. and Acinetobacter spp., enterococci and Vibrio cholerae. The procedure is modified for testing fastidious species; i.e., H. influenzae, N. gonorrhoeae and S. pneumoniae and other streptococci. Mueller Hinton II Agar is manufactured to contain low levels of thymine and thymidine,and controlled levels of calcium and magnesium, Thymine and thymidine levels of raw materials are determined using the disc diffusion procedure with trimethoprim-sulfamethoxazole (SXT) discs and Enterococcus faecalis ATCC 29212. Calcium and magnesium levels are controlled by testing raw materials and supplementing with sources of calcium and/or magnesium as required to produce correct zone diameters with aminoglycoside antibiotics and Pseudomonas aeruginosa ATCC 27853.
Intended Use
For antimicrobial disc diffusion susceptibility testing of common, rapidly growing bacteria by the Bauer-Kirby method, as standardised by the Clinical and Laboratory Standards Institute (CLSI).
Product Information
Category
Catalog Number
FP90M2001, FP90M2002
Pack Size
10 per pack